Cloning, expression and rapid purification of recombinant chicken interferon-alpha

The poultry industry in Vietnam is vulnerable to many viral diseases. Since antibiotics and vaccination provide inadequate protection, it will be beneficial have alternative immunostimulants boost non-specific immunity poultry. Chicken interferon-alpha (ChIFNα) has been described previously with antiviral activity against pathogens. Therefore, the purpose of this study clone express recombinant ChIFNα Escherichia coli. gene was successfully cloned into pET32a(+) vector, then expressed E. coli Rosetta strain. Different expression conditions were tested for best yield protein. Results showed that as inclusion bodies a 30 mg/100 L culture after induction 0.5 mM IPTG 4 hours at 37 oC. protein purified using affinity column chromatography under denaturing purity > 94%. Western blot analysis indicated reacted specifically its antibody. Further studies carried out characterize biological activities application industry.